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MICROBIOLOGIA
Lorenzo Drago
Microbiologia Clinica, Dipartimento di Scienze Cliniche “L.Sacco”, Universita’ di Milano
Conclusioni
Secondo alcuni Autori, tutti questi metodi e/o criteri, dovrebbero rappresentare dei point-of-care iniziali, dove viene ribadita la necessita’ di meglio caratterizzare quali-quantitativamente le eventuali specie isolate con metodi colturali o di biologia molecolare. Anche alcuni metodi cromogenici (BVBlue Test) che misurano le sialidasi, enzimi prodotti dalla flora mista ritenuta responsabile di vaginosi batterica, potenzialmente utilizzabili in alternativa alle indagini microscopiche e morfometriche, non risolvono il problema della valutazione quali-quantitativa dal punto di vista microbiologico.
Un approccio nuovo e promettente sono i test di biologia molecolare per la diagnosi di vaginosi. Cominciano a comparire, infatti, in letteratura nuovi approcci metodologici, tra questi i piu’ affidabili e rapidi, rispetto all’indagine microscopica, risultano essere i test di ibridizzazione del DNA, ma soprattutto la Real Time PCR, che consente di rilevare rapidamente e contemporaneamente i principali microrganismi correlati alla vaginosi.
Approfondimenti
Evaluation of a Point-of-Care
Test, BVBlue, and Clinical and Laboratory Criteria for Diagnosis of
Bacterial Vaginosis
C. S. Bradshaw A. N. Morton S. M. Garland, L. B. Horvath, I. Kuzevska
and C. K. Fairley
The effectiveness of
live lactobacilli in combination with low dose oestriol (Gynoflor) to
restore the vaginal flora after treatment of vaginal infections
Erdinc Ozkinay, Mustafa Cosan Terek, Murat Yayci, Renato Kaiser,
Philipp Grob, Gungor Tuncaya
Strategies for diagnosis of
bacterial vaginosis in a resource-poor setting
S F Posner PhD J Kerimova MD PhD, F Aliyeva MD PhD and A Duerr MD PhD
Drago L, De Vecchi E, Nicola L, Colombo A, Gismondo MR.
Microbiological evaluation of commercial probiotic products available in
Italy.
J Chemother.
2004 Oct;16(5):463-7.
PMID: 15565913 [PubMed - indexed for MEDLINE]
Drago L, De Vecchi E, Nicola L, Valli M, Gismondo MR.
Effects of subinhibitory concentrations of ibuprofen isobuthanolammonium
on virulence factors of uropathogenic Escherichia coli.
J Chemother.
2002 Jun;14(3):314-5. No abstract available.
PMID: 12120890 [PubMed - indexed for MEDLINE]
Drago L, De Vecchi E, Fassina MC, Mombelli B, Bonaccorso C, Gismondo MR.
Antimycotic activity and phagocytosis effects of econazole in
combination with ibuprofen isobuthanolammonium against vaginal strains.
J Chemother.
2000 Dec;12(6):509-15.
PMID: 11154035 [PubMed - indexed for MEDLINE]
Drago L, Mombelli B, De Vecchi E, Bonaccorso C, Fassina MC, Gismondo MR.
Candida albicans cellular internalization: a new pathogenic factor?
Int J
Antimicrob Agents. 2000 Dec;16(4):545-7.
PMID: 11118877 [PubMed - indexed for MEDLINE]
Drago L, Gismondo MR, Lombardi A, de Haen C, Gozzini L.
Inhibition of in vitro growth of enteropathogens by new Lactobacillus
isolates of human intestinal origin.
FEMS
Microbiol Lett. 1997 Aug 15;153(2):455-63.
PMID: 9271875 [PubMed - indexed for MEDLINE]
Sha BE, Zariffard MR, Wang QJ, Chen HY, Bremer J, Cohen MH, Spear GT.
Female genital-tract HIV load correlates inversely
with Lactobacillus species but positively with bacterial vaginosis and
Mycoplasma hominis.
J Infect Dis. 2005 Jan 1;191(1):25-32. Epub 2004 Dec 02.
PMID: 15592999 [PubMed - indexed for MEDLINE]
Chaudry AN, Travers PJ, Yuenger J, Colletta L, Evans P, Zenilman JM, Tummon A.
Analysis of vaginal acetic acid in patients
undergoing treatment for bacterial vaginosis.
J Clin Microbiol. 2004 Nov;42(11):5170-5.
PMID: 15528711 [PubMed - indexed for MEDLINE]
Brown HL, Fuller DD, Jasper LT, Davis TE, Wright JD.
Clinical evaluation of
affirm VPIII in the detection and identification of Trichomonas
vaginalis, Gardnerella vaginalis, and Candida species in vaginitis/vaginosis.
Infect Dis Obstet Gynecol.
2004;12(1):17-21.
PMID: 15460191 [PubMed - indexed for MEDLINE]
Cytolytic vaginosis: misdiagnosed as candidal
vaginitis.
Infect Dis Obstet Gynecol. 2004;12(1):13-6.
PMID: 15460190 [PubMed - indexed for MEDLINE]
Geisler WM, Yu S, Venglarik M, Schwebke JR.
Vaginal leucocyte counts in women with bacterial
vaginosis: relation to vaginal and cervical infections.
Sex
Transm Infect. 2004 Oct;80(5):401-5.
PMID: 15459411 [PubMed - indexed for MEDLINE]
Reid G, Burton J, Hammond JA, Bruce AW.
Nucleic acid-based diagnosis of bacterial vaginosis
and improved management using probiotic lactobacilli.
J Med Food.
2004 Summer;7(2):223-8.
PMID: 15298771 [PubMed - indexed for MEDLINE]
Alvarez-Olmos MI, Barousse MM, Rajan L, Van Der Pol BJ, Fortenberry D, Orr D, Fidel PL Jr.
Vaginal lactobacilli in adolescents: presence and
relationship to local and systemic immunity, and to bacterial vaginosis.
Sex
Transm Dis. 2004 Jul;31(7):393-400.
PMID: 15215693 [PubMed - indexed for MEDLINE]
Dicker LW, Mosure DJ, Steece R, Stone KM.
Laboratory tests used in US public health
laboratories for sexually transmitted diseases, 2000.
Sex
Transm Dis. 2004 May;31(5):259-64.
PMID: 15107626 [PubMed - indexed for MEDLINE]
Tokyol C, Aktepe OC, Cevrioglu AS, Altindis M, Dilek FH.
Bacterial vaginosis:
comparison of Pap smear and microbiological test results.
Mod Pathol. 2004 Jul;17(7):857-60.
PMID: 15073605 [PubMed - indexed for MEDLINE]
Impact
of bacterial vaginosis on detection of chlamydial infection.
Int J STD AIDS.
2004 Mar;15(3):209-10. No abstract available.
PMID: 15038873 [PubMed - indexed for MEDLINE]
Anderson MR, Klink K, Cohrssen A.
Evaluation of vaginal complaints.
JAMA.
2004 Mar 17;291(11):1368-79. Review.
PMID: 15026404 [PubMed - indexed for MEDLINE]
Boskey ER, Atherly-Trim SA, O'Campo PJ, Strobino DM, Misra DP.
Acceptability of a
self-sampling technique to collect vaginal smears for gram stain
diagnosis of bacterial vaginosis.
Womens Health
Issues. 2004 Jan-Feb;14(1):14-8. Erratum in: Womens Health Issues. 2004
Mar-Apr;14(2):69. Misra, P [corrected to Misra, Dawn P].
PMID: 15001184 [PubMed - indexed for MEDLINE]
Zarakolu P, Sahin Hodoglugil NN, Aydin F, Tosun I, Gozalan A, Unal S.
Reliability of
interpretation of gram-stained vaginal smears by Nugent's scoring system
for diagnosis of bacterial vaginosis.
Diagn Microbiol
Infect Dis. 2004 Feb;48(2):77-80.
PMID: 14972374 [PubMed - indexed for MEDLINE]
Light microscopy observation of lytic enzymatic
activity of the organisms associated with bacterial vaginosis.
Cent Eur J Public Health. 2003 Dec;11(4):238-9.
PMID: 14768789 [PubMed - indexed for MEDLINE]
Larsson PG, Carlsson B, Fahraeus L, Jakobsson T, Forsum U.
Diagnosis of bacterial vaginosis: need for validation
of microscopic image area used for scoring bacterial morphotypes.
Sex
Transm Infect. 2004 Feb;80(1):63-7.
PMID: 14755040 [PubMed - indexed for MEDLINE]
Yen S, Shafer MA, Moncada J, Campbell CJ, Flinn SD, Boyer CB.
Bacterial vaginosis in
sexually experienced and non-sexually experienced young women entering
the military.
Obstet Gynecol. 2003 Nov;102(5 Pt
1):927-33.
PMID: 14672465 [PubMed - indexed for MEDLINE]
Nelson DB, Bellamy S, Gray TS, Nachamkin I.
Self-collected versus provider-collected vaginal
swabs for the diagnosis of bacterial vaginosis: an assessment of
validity and reliability.
J Clin
Epidemiol. 2003 Sep;56(9):862-6.
PMID: 14505771 [PubMed - indexed for MEDLINE]
Review of cytologic
criteria of bacterial vaginosis: examination of 2,841 Papanicolaou-stained
vaginal smears.
Diagn Cytopathol. 2003 Sep;29(3):156-9.
PMID: 12951684 [PubMed - indexed for MEDLINE]
West B, Morison L, van der Loeff MS, Gooding E, Awasana AA, Demba E, Mayaud P.
Evaluation of a new rapid diagnostic kit (FemExam)
for bacterial vaginosis in patients with vaginal discharge syndrome in
The Gambia.
Sex
Transm Dis. 2003 Jun;30(6):483-9.
PMID: 12782948 [PubMed - indexed for MEDLINE]
questa pagina è aggiornata al 10/05/2005
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